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Manav Jain's avatar

Great article! From what you have read, how might error rates and fidelity of pipolB compare to conventional high-fidelity polymerases, and what implications would that have for downstream sequencing?

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Juraj's avatar

Great article, thx for that.

You also mentioned RNA and the necessity of RT, are we sure that all RNA in the sample is completely transcribed into DNA? Random primers work great for standard samples but how does it look for META samples where there should be more RNA in addition to a lot of DNA?

And the second question, can metagenomic samples be prepared in a way that would remove the most represented (and therefore probably already identified) DNA molecules, i.e. to leave only what standard methods have a problem with in the sample

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